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Double-label immunofluorescence for ubiquitin (green) and FUS (red) in NIFID. Merged images show cell nuclei stained with DAPI (blue). Ubiquitin-positive neuronal cytoplasmic inclusions (NCIs) and vermiform neuronal intranuclear inclusions (NIIs, arrows) always show strong labeling for FUS (a, b). Occasionally, FUS-positive NCIs do not label for ubiquitin (red NCI in merged image, b). A round NII showing colocalization of ubiquitin and FUS. Scale bar = 15 μm (a, b); 10 μm (c).
