Table 1.

Primer sequences and optimal conditions used in PCR analysis.

Gene	Sense primer sequence (5′–3′)	Antisense primer sequence (5′–3′)	Amplification conditions	Amplification cycles, n
SAA3	AGC CTT CCA TTG CCA TCA TTC TT	AGT ATC TTT TAG GCA GGC CAG CA	94°C 1min; 58°C 1min; 74°C 1min	25
IP10	GTGTTGACATCATTGCCACG	GCTTACAGTACAGAGCTAGG	95°C 30 sec; 60°C 30 sec; 45°C 45 sec	30
CCL-2	CCCCACTCACCTGCTGCTACT	CACTGTCACACTGGTCACTCC	95°C 50 sec; 64°C 50 sec; 72°C 1 min	36
CCL-3	ACTGCCCTTGCTGTTCTTCTCT	AGGCATTCAGTTCCAGGTCAGTGA	95°C 30 sec; 61°C 30 sec; 72°C 30 sec	33
CCL-4	CCCTCTCTCTCCTCTTGCTCGT	TTCAACTCCAAGTCACTCATGTACTCA	94°C 30 sec; 55°C 30 sec; 72°C 1 min	32
CXCL-2	GCTGTCAATGCCTGAAGACC	TAGTTCCCAACTCACCCTCTC	95°C 50 sec; 65°C 50 sec; 72°C 1 min	36
IL-1β	AAGGAGAACCAAGCAACGAC	GAGATTGAGCTGTCTGCTCA	95°C 50 sec; 63°C 50 sec; 72°C 1 min	34
CCR-2	CACGAAGTATCCAAGAGCTT	CATGCTCTTCAGCTTTTTAC	94°C 30 sec; 58°C 45 sec; 72°C 70 sec	35
CCR-5	TTCCCTGTCATCGCTTGCTCT	CGGATGGAGATGCCGATTTT	94°C 1 min; 60°C 1 min; 72°C 2 min	40
IL-1ra	GACCCTGCAAGATGCAAGCC	CAGGACGGTCAGCCTCTAGT	95°C 20 sec; 51°C 20 sec; 72°C 20 sec	36
18S	GTA ACC CGT TGA ACC CCA TT	CCA TCC AAT CGG TAG TAG CG	95°C 30 sec; 59°C 30 sec; 72°C 30 sec	24