Figure 3.
Cholinergic drive of fictive respiration in preparations from (a) control and (b) nicotine-exposed mice. Changes in amplitude and frequency of fictive respiration (fR) after the application of 100 µM atropine (atrop), or 100 µM hexamethonium (hexam) or 100 µM neostigmine (neost) in en bloc (open bars) and slice (filled bars) preparations. *p < 0.05, when compared with the basal response (Wilcoxon test). Dotted lines indicate the basal amplitude and frequency (100%). The number of neonates is indicated inside bars.