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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Transfusion. 2009 Aug 4;49(12):2686–2699. doi: 10.1111/j.1537-2995.2009.02333.x

Figure 5. Cell conjugation is defective in Mirasol treated cells.

Figure 5

Normal PBMCs were primed with γ-irradiated cells to enrich for allo-specific cells. These responder cells were labeled with PKH red, and cultured with stimulators stained with PKH green. Stimulators were either γ-irradiated or Mirasol treated cells from the same donor as the priming cells. Representative sample plots (A–B) from t=2 hours show cells gated on red responder events, with the green stimulator events plotted as histograms. The markers denote the percentage of responders bound to stimulators. Four replicate samples were tested longitudinally from 2 – 8 hours (C). A minimum of 4,000 red events were collected for each sample. Error bars represent 95% confidence intervals.