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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: J Virol Methods. 2005 Nov 28;132(1-2):195–203. doi: 10.1016/j.jviromet.2005.10.014

Fig. 1.

Fig. 1

Comparison of the transfection and infection efficiency of HCV replication system using vaccinia-T7 and adeno-T7 vectors: (A) after transfection with the pOS8 plasmid, cells were infected with virus vectors using control adenovirus (Ad-Psi5), recombinant adeno-T7 polymerase (Ad-T7pol), or vaccinia-T7 polymerase (vTF7-3) at an MOI of 10. (B) RPA for negative-strand HCV RNA was performed with H77 plasmid as a positive control. (C)Western blotting for HCV core protein was performed on CV-1 cell lysates 24 h after infection. (D) Quantitative HCV core ELISA results indicated that an MOI of 10 was optimal for adenoviral-driven HCV protein production.