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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: J Virol Methods. 2005 Nov 28;132(1-2):195–203. doi: 10.1016/j.jviromet.2005.10.014

Fig. 2.

Fig. 2

(A) A control plasmid was prepared by mutating the active site motif from GDD to AAG in the NS5B RNA-dependent RNA polymerase sequence (H77GDD→AAG). (B) A qualitative strand-specific RT-PCR for negative-strand HCV RNA was performed as previously described (Lanford et al., 1995). (C)Western blotting analysis demonstrated that transfection/infection with H77 + Ad-T7-pol also resulted in HCV core protein production. (D) Ribonuclease protection assay demonstrated detectable negative-strand HCV RNA in CV-1, Huh7, and Huh-T7 cell lines on day 2.