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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Anal Chem. 2010 May 1;82(9):3636–3641. doi: 10.1021/ac902877v

Figure 1.

Figure 1

Schematic showing gradient sample pumping, μFFE separation and on-chip LIF detection of analytes. A LabView computer program controlled the flow rates of pumps 2 and 3 to generate a concentration gradient of ligand. Sample was pumped into the separation channel through an access hole (2). Separation buffer was introduced into the chip via a hole (1) and carried the sample toward the exit holes (3). A separation potential was applied to the electrodes (4) to achieve analyte separation (green lines). A laser beam was expanded into a line (5) and projected across the separation channel. LIF detection was performed via microscope with CCD camera positioned perpendicular to the plane of the page.