FIG. 2.

Detection of two common integrants in FACS-sorted granulocyte and CD3⁺ lymphocyte subsets. Granulocyte and CD3⁺ lymphocyte subsets were sorted from PBMCs from dog G206, using subset-specific antibodies. DNA was prepared from the collected cells and subjected to PCR analysis (see Materials and Methods) using either two vector-specific primers (Vector) or one vector-specific primer in combination with integrant-specific primers (see Materials and Methods, Subset Sorting). Lane 1, unsorted PBMCs from G206; lane 2, G206 granulocyte subset; lane 3, G206 CD3 lymphocyte subset; lane 4, PBMCs from a nontransplanted animal; lane 5, water control.