FIG. 3.

Polyclonal hematopoietic repopulation after transplantation with lentivirus-transduced CD34-enriched cells. Peripheral blood samples from (A) dog G206, (B) dog G236, and (C) dog G293 at various time points after transplantation were analyzed by LAM-PCR, showing that multiple different clones contribute to the repopulation. The number above each lane indicates how many days after transplantation (DPT) the DNA was prepared from the PBMCs of each dog. For G206, LAM-PCRs were carried out in duplicate. The arrows in (B) indicate the 155- and 250-bp fragments cloned from G236 DNA (see Results).