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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: J Gen Virol. 2008 Aug;89(Pt 8):1978–1986. doi: 10.1099/vir.0.2008/000778-0

Fig. 2. Inhibition of hMPV-induced interferon-β and chemokines gene transcription by expression of dominant negative RIG-I.

Fig. 2

A549 cells were cotransfected with a luciferase reporter plasmid containing INF-β (A), RANTES (B) or IL-8 (C) promoter together with a RIG-I dominant negative mutant expression plasmid (RIG DN), or the corresponding empty vector (EV). Cells were infected with hMPV and harvested at 15 or 24 h p.i to measure luciferase activity. Uninfected plates served as controls. For each plate luciferase was normalized to the β-galactosidase reporter activity. Data are expressed as mean ± standard deviation of normalized luciferase activity. Bars represent the mean value of triplicate samples of one experiment, n = at least two experiments. *, P< 0.05 relative to hMPV-infected EV-treated cells.