. Author manuscript; available in PMC: 2010 Aug 14.

Published in final edited form as: J Mol Biol. 2009 Jun 6;391(2):450–460. doi: 10.1016/j.jmb.2009.05.085

Table 1.

Summary of equilibrium and kinetic data for the three-state RNases H. The values in parentheses indicate the standard deviation calculated from at least three separate denaturant melts.

			Periphery		Core		Periphery	Core
		RNH3^*	I25A RNH3	F8A RNH3	Q105G RNH3	W85A RNH3	F8A	W85A
Δ G _{urea melt} (kcal mol ⁻¹ )		13	8.2 (0.4)	10.0 (0.2)	10.6 (0.5)	11.3 (0.5)	6.5 (0.5)	6.8 (0.5)
m_{eq urea melt} (kcal mol ⁻¹ M⁻¹ )		2.1	2.2 (0.1)	2.14 (0.03)	2.11 (0.05)	2.24 (0.1)	1.9 (0.2)	1.9 (0.1)
Δ G _{fixed m} (kcal mol ⁻¹ )			7.86 (0.07)	9.78 (0.07)	10.5 (0.2)	10.56 (0.06)	7.05 (0.08)	7.5 (0.1)
Δ G _kinetic (kcal mol ⁻¹ )		13.4	8.1	11.02	11.9	11.67	9.22	8.6
m _kinetic (kcal mol ⁻¹ M⁻¹ )		2.25	2.13	2.35	2.43	2.37	2.7	2.48
k_in (H ₂O) (s ⁻¹ )		1.89	0.37	0.55	0.59	0.48	0.227	0.1514
m_in (kcal mol ⁻¹ M⁻¹ )		0.28	0.49	0.50	0.15	0.19	0.647	0.415
k_ni (H ₂O) (s ⁻¹ )		1.5E-07	4.4E-05	2.6E-06	5.5E-08	1.6E-07	9.50E-05	2.50E-05
m_ni (kcal mol ⁻¹ M⁻¹ )		−0.48	−0.49	−0.48	−0.60	−0.51	−0.529	−0.472
K_ui		585	110	611	54	133	2580	350
m_ui (kcal mol ⁻¹ M⁻¹ )		1.50	1.15	1.37	1.68	1.66	1.505	1.596
Δ G_ui (kcal mol ⁻¹ )		3.76	2.77	3.79	2.35	2.89	4.64	3.46
ϕ ^I	1		0.19	−0.01	0.56	0.36	−0.42	0.04
	2		0.2	0.0	0.6	0.5	−0.1	0.0
	3		0.19	−0.01	0.94	0.51	−1.64	0.07
ϕ ^t.s.	1		0.35	0.47	1.23	0.97	0.51	0.78
	2		0.3	0.4	1.2	1.0	0.4	0.5
	3		0.37	0.29	1.39	0.96	−0.93	0.62
β _T	1	0.77	0.77	0.77	0.71	0.76	0.75	0.78
	2	0.8	0.8	0.8	0.7	0.8	0.7	0.8
	3	0.79	0.77	0.80	0.75	0.78	0.80	0.81

These values were previously determined by Raschke, Kho, and Marqusee.²⁶

1. calculated using a fixed m-value of 2.1

2. calculated using the m-value from urea melts

3. calculated using the m-value from the kinetic data; m_kinetic = m_ui + m_in − m_ni