TABLE 1.
Biochemical and biophysical parameters of Hsp90 oligomers
| Techniques | Parameters | Dimer | Type I | Type II | Type III |
|---|---|---|---|---|---|
| Ultracentrifugation (sucrose gradient) | (S) | 7 | 11 | 16 | >19 |
| Size exclusion chromatography | (kDa)a | 415 | 638 | 863c | 1060c |
| Rs (Å) | 59 | 77 | 85–90c | NDd | |
| Analytical ultracentrifugation | |||||
| Velocity | S20,w0 (S) | 6.86 ± 0.05b | 10.4 ± 0.3 | 15.0 ± 0.8 | 17.7 ± 2.0 |
| f/f0 | 1.25b | 1.50 ± 0.03 | 1.58 ± 0.06 | ND | |
| (kDa) | 355 ± 18 | 618 ± 57 | ND | ||
| Equilibrium | (kDa) | 144–175b | 316 ± 18 | 539 ± 67 | ND |
| Multiangle laser light-scattering | (kDa) | 189.6 ± 1.8 | 364.7 ± 3.7 | 587.9 ± 30.7 | >900e |
| Rg (Å) | 92 | 122 | 185 | 234 | |
| High mass MALDI-TOF MS | (kDa) | 169.9 ± 0.5 | 341.2 ± 1.1 | 511.9 ± 3.1 | 1026.8 ± 21.9 |
a Apparent molecular mass considering a globular protein.
b From Garnier et al. (27). f and f0 are the frictional coefficients.
c Extrapolation from calibration curve. Rs is the Stokes radius. S20,w0 is the corrected sedimentation coefficient at 20 °C in water at infinite dilution.
d ND, not determinable.
e Minimal molecular mass measured. Rg is the gyration radius.