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. 2010 Mar 15;285(20):14871–14881. doi: 10.1074/jbc.M109.069591

FIGURE 6.

FIGURE 6.

Subcellular targeting of K299A, R301A, K303A, or K299A/R301A mutated APE1 observed by laser confocal microscopy. K299A, R301A, K303A, or K299A/R301A mutated APE1 vectors are based on the 289–318 truncated APE1 expression vectors with C-terminally tagged EGFP. Subcellular localization of different truncated APE1 proteins in HeLa cells was observed by laser confocal microscopy at 48 h after transfection. Scale bar, 10 μm. The Western blot against GFP antibody in nuclear (N), cytosolic (C), and mitochondrial (M) enriched fractions are also shown on the right. The bands represent distribution of the exogenously expressed mutant in each subcellular organelle.