FIGURE 5.

Neutralization of IL-31 native form by OSMR-l-GPL. A, secretion of native IL-31 was measured in human cell lines by ELISA as indicated under “Experimental Procedures.” Error bar represents S.D. B, native IL-31 was immunopurified on an AN-C2 anti-IL-31 monoclonal antibody column and analyzed by SDS-PAGE and silver staining. C, after a 2-h contact between recombinant glycosylated IL-31 or native IL-31 (0.4 nm) with increasing concentrations of OSMR-L-GPL, the mixture was added to the Go-G-UVM cell line for 10 min. The STAT3 tyrosine phosphorylation levels were determined by Western blot analysis.