FIGURE 6.

Comparison of OSMR-L-GPL with other inhibitors. A, IL-31 (2 nm) was preincubated with 15 nm of the soluble receptors sGPL or sOSMR, or a combination of both, or OSMR-L-GPL for 2 h at 37 °C. Mixed reagents were added to the Go-G-UVM cell line for 10 min at 37 °C. The STAT3 tyrosine phosphorylation levels were determined by Western blot analysis. B, following a preincubation of IL-31 (0.4 nm) with increasing concentrations of the purified OSMR-L-GPL or a polyclonal neutralizing anti-IL-31 antibody for 2 h at 37 °C, the Go-G-UVM cell line was activated for 10 min at 37 °C. The STAT3 phosphorylation levels were determined by Western blot analysis.