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. 2010 Mar 12;285(20):14909–14919. doi: 10.1074/jbc.M110.106724

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — WT and mutant cochlin oligomerization in UB/UE-1 cells. UB/UE-1 cells were cotransfected with HA- and FLAG-tagged WT and mutant G90E cochlins as indicated at a 1:1 ratio. Cochlin was detected by immunoprecipitation (IP) using anti-FLAG antibody, and the Western blot (WB) was probed with anti-LCCL antibody after 8% nonreducing SDS-PAGE (A). Also shown is a control Western blot probed with anti-LCCL antibody after nonreducing SDS-PAGE of the cell lysates shown in A (B). Vec, vector; DI-Coch, dimer cochlin; OL-Coch, oligomer cochlin; FL-Coch, full-length cochlin.