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mTOR inhibitors cause nuclear accumulation of Maf1. MG63 cells were treated with 0.5 μm CCI-779 or 0.5 μm WYE-132 for 6 h. Merge with DAPI shows the localization of Maf1 in the nucleus. Left, cells were analyzed by immunofluorescence confocal microscopy using Maf1 antibody. Right, nuclei were counterstained with DAPI. Red, Alexa Fluor 594; Blue, DAPI.
