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. 2010 Mar 12;285(20):15324–15332. doi: 10.1074/jbc.M110.110635

FIGURE 2.

FIGURE 2.

Adipocyte differentiation is accompanied by down-regulation of CRABP-II expression. a, preadipocytes were induced to differentiate, and CRABP-II expression at denoted time points was analyzed by Q-PCR. b, immunoblots of CRABP-II expression in preadipocytes at the denoted time points following differentiation induction. c, preadipocytes were treated with actinomycin D (5 μg/ml) for denoted time points, and levels of CRABP-II mRNA were analyzed by Q-PCR. d and e, preadipocytes were treated with insulin (10 μg/ml), dexamethasone (dex) (0.25 mm), or IBMX (0.5 mm) for 4 h. Levels of CRABP-II mRNA were analyzed by Q-PCR (d) and CRABP-II protein levels assessed by immunoblots (e). Error bars indicate S.D.