FIGURE 3.

Insulin, dexamethasone, and IBMX down-regulate CRABP-II expression. a and b, preadipocytes were pretreated with denoted concentrations of wortmannin (wort) (a) or with RU486 (b) and then treated with 10 μg/ml insulin (a) or 250 nm dexamethasone (b). CRABP-II mRNA expression was analyzed by Q-PCR. c, preadipocytes were treated with denoted concentrations of IBMX, and CRABP-II mRNA expression was analyzed by Q-PCR. d, preadipocytes were treated with either actinomycin D (act D) (5 μg/ml) or IBMX (0.5 mm) for the denoted times, and CRABP-II mRNA expression was analyzed by Q-PCR. Error bars indicate S.D.