FIGURE 6.

CRABP-II is directly repressed by GR. a, preadipocytes were pretreated with cycloheximide (CHX) (10 μg/ml, 15 min) and then treated with dexamethasone (dex) for 4 h. CRABP-II expression at the denoted time was assessed by Q-PCR. b, immunoblot of GR in preadipocytes infected with empty lentivirus (ev) or with lentivirus harboring GR shRNA. c and d, preadipocytes were infected with lentoviruses and treated with dexamethasone for 4 h (c) or 12 h (d). Expression levels of CRABP-II mRNA (c) and protein (d) were assessed by Q-PCR and immunoblots, respectively. e, GR half-site in the CRABP-II promoter. Error bars indicate S.D. f, ChIP assays indicating recruitment of GR to the GRE of the CRABP-II gene following treatment with dexamethasone (see “Experimental Procedures” for assay details). IP, immunoprecipitation.