FIGURE 7.

C/EBPα represses CRABP-II in mature adipocytes. a, five C/EBPα sites are located in the CRABP-II promoter. b, top, ChIP assays indicate recruitment of C/EBPα to the promoter region containing the putative C/EBPα sites in mature adipocytes. Bottom, ChIP assays indicating that in mature adipocytes CREM does not occupy the Cre in the CRABP-II promoter (compare with Fig. 5j). c and d, expression of C/EBPα in mature adipocytes was decreased using a lentivirus harboring C/EBPα shRNA. CRABP-II expression was analyzed by Q-PCR (b) or immunoblot (c). IP, immunoprecipitation. Error bars indicate S.D.