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. 2010 Mar 19;285(20):14999–15009. doi: 10.1074/jbc.M110.109819

FIGURE 3.

FIGURE 3.

A20 requires TAX1BP1 to terminate antiviral signaling. A, 293T cells were transfected with the indicated plasmids (1 μg each). Co-IP and immunoblots were performed with the indicated antibodies. B, IKKi was immunoprecipitated from whole cell lysates derived from mock-infected and virally infected wild-type MEFs. Bound proteins were detected via immunoblotting, and lysates were probed as indicated. C, Tax1bp1+/− or Tax1bp1−/− MEFs were mock-infected or virally infected for 16 h, and lysates were subjected to immunoprecipitation and/or immunoblotting using the indicated antibodies. D, 293T cells were transfected with either control scrambled siRNA or TAX1BP1 siRNA. After 24 h, cells were transfected with IFNβ luc (100 ng) and pRL-tk (10 ng) DNA and plasmids encoding IKKi (1 μg) and A20 (200 ng) as indicated. E, Tax1bp1+/− or Tax1bp1−/− MEFs were transfected with an IFNβ luc reporter (200 ng) and pRL-tk (20 ng) and a plasmid encoding A20 (1 μg) as indicated. After 36 h, cells were either mock-infected or infected with virus for 16 h, followed by luciferase assays. IB, immunoblot; IP, immunoprecipitation. Error bars, S.E.