FIGURE 2.

Isolation and structural characterization of the antigenic 2-nonenal-lysine adducts. A, competitive ELISA analysis of HPLC fractions for immunoreactivity with mAb 27Q4. The reaction was performed by incubating 200 mm N^α-acetyllysine with 200 mm 2-nonenal in 1 ml of 50 mm sodium phosphate buffer, pH 7.2, at 37 °C for 24 h. Solid line, profile of UV absorbance at 200–650 nm. Bar, competitive ELISA analysis. B, chemical structures of cis-HHP-lysine and (P-1) and trans-HHP-lysine (P-2). C, competitive ELISA analysis with cis- and trans-HHP-lysine. Competitors are as follows: closed circle, N^α-acetyl-trans-HHP-lysine (P-2); closed triangle, N^α-acetyl-cis-HHP-lysine (P-1); open circle, N^α-acetyllysine.