TABLE 1.
Summary of 3-OMG transport in bEnd.3 cells
| Zero-trans uptakea |
Equilibrium exchange uptakeb |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Kmc | 95% confidence intervalsc | Relative Vmaxc | 95% confidence intervalsc | nd | Km | 95% confidence intervalsc | Relative Vmaxc | 95% confidence intervalsc | nd | |
| Control | 6.7 ± 0.4 | 5.1 to 8.4 | 1 | 0.9 to 1.1 | 12 | 14.9 ± 12.6 | −62.2 to 99.9 | 3.17 ± 0.46e | −3.1 to 9.5 | 3 |
| CN | 18.7 ± 6.7 | −10.5 to 47.9 | 7.32 ± 1.2e,f | 2.1 to 12.6 | 6 | 18.4 ± 13.9 | −41.4 to 78.3 | 10.0 ± 3.5e | −4.8 to 24.9 | 3 |
| FCCP | 30.1 ± 18.4 | −48.9 to 109.2 | 4.26 ± 1.4 | −1.8 to 10.2 | 6 | 35.9 ± 27.8 | −83.9 to 155.8 | 14.6 ± 6.5e | −13.5 to 42.6 | 3 |
| Glucose deprivation | 29.4 ± 11.0 | −8.6 to 20.5 | 1.7 ± 0.3e | 0.5 to 2.7 | 3 | |||||
| AICAR | 8.4 ± 4.0 | −8.8 to 25.7 | 2.0 ± 0.3e | 0.6 to 3.4 | 4 | |||||
a Unidirectional sugar uptake into cells was depleted of sugar at 4 °C for 15 min.
b Unidirectional sugar uptake was into cells where [3-OMG]i = [3-OMG]o.
c The concentration dependence of sugar uptake was analyzed by nonlinear regression assuming Michaelis-Menten kinetics to obtain Km and Vmax. All Vmax values are expressed relative to Vmax for zero-trans uptake in matched control cells. Control zero-trans 3-OMG uptake Vmax = 180 pmol/μg/min. Results are shown as mean ± S.E., and the 95% confidence intervals for the analysis are indicated.
d Number of separate experiments in which 3-OMG transport was measured was at least four different [3-OMG].
e Vmax is significantly greater than control Vmax for zero-trans uptake (p < 0.05).
f CN stimulation of Vmax for 3-OMG zero-trans uptake is 3.18 ± 0.92-fold greater than FCCP stimulation of uptake.