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. 2010 Mar 15;285(20):15557–15565. doi: 10.1074/jbc.M109.082578

FIGURE 3.

FIGURE 3.

The effect of KCa1. 3 channel inhibitors on HlyA-induced volume changes and lysis in human erythrocytes. A, the effect of clotrimazole (CLT, 20 μm, filled circles) on the changes in forward scatter induced by HlyA (3 ng ml−1) compared with controls (open squares). B, shows the effect of TRAM-34 (10 μm, filled triangles) on the change in forward scatter induced by HlyA (3 ng ml−1) compared with controls (open squares). C and D, the corresponding changes in side scatter in response to clotrimazole and TRAM-34 are shown in C and D, respectively. All of the above values are normalized to time 0 and given as mean values ± S.E. (n = 5–6). The hemolysis was measured as the level of released hemoglobin in the absence or presence of increasing concentrations of clotrimazole (E) and TRAM-34 (F) at two concentrations of HlyA leading to 20 and 50% lysis after 60 min (5 and 10 ng ml−1, respectively). The values are given as the means ± S.E. (n = 4–6).