FIGURE 2.

Transport activity of hSERT Cys mutants. C109A and hSERT Cys mutant cDNAs were transiently transfected in HEK-293 cells as described under “Experimental Procedures.” Forty-eight hours post-transfection, the cells were assayed for [³H]5-HT transport activity. Nonspecific uptake was determined using 10 μm fluoxetine and typically represented between 3 and 5% of total uptake. In the case of the Q332C mutant, which demonstrated the lowest specific uptake, total uptake was 12,560 ± 210 cpm, and nonspecific uptake was 650 ± 20 cpm. The results represent means ± S.E. (error bars) from at least three separate experiments performed in triplicate.