FIGURE 5.

Protection against MTS reactivity by SERT substrates. The ability of 5-HT (A), MDMA (B), and MPP⁺ (C) to protect the mutants from MTS reactivity was evaluated in HEK-293 cells transiently transfected with hSERT Cys mutants. Briefly, the cells were incubated with buffer, 20 μm 5-HT, 20 μm MDMA, or 200 μm MPP⁺ for 5 min at room temperature, followed by incubation in the presence or absence of 1 mm MTSET for 10 min. After washing, [³H]5-HT uptake assays were performed as described under “Experimental Procedures.” Bars, mean ± S.E. (error bars) from three independent experiments performed in triplicate. The data were normalized to the total uptake of each individual mutant. *, p < 0.01 versus MTSET treatment using a one-way analysis of variance with Bonferroni's post hoc test.