FIGURE 9.

Effects of SERT ligands on reactivity of bifunctional MTS reagents at hSERT TMH VI and XI Cys double mutant. HEK-293 cells transiently expressing the hSERT Cys single mutants or Cys double mutant were incubated with 100 μm MTS-3-MTS for 10 min at room temperature in the presence or absence of 20 μm 5-HT (A) or 50 μm cocaine (B) (5-min preincubation). Following incubation, the cells were washed twice and assayed for [³H]5-HT uptake as described under “Experimental Procedures.” The data were normalized to the specific uptake of the untreated control for each individual mutant. Data represent the mean ± S.E. (error bars) from three independent experiments performed in triplicate. *, p < 0.05 using a t test compared with MTS-3-MTS alone.