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. 2010 Mar 19;285(20):15399–15407. doi: 10.1074/jbc.M109.055111

FIGURE 4.

FIGURE 4.

Enzymatic properties of recombinant INV-E and INV-E:C294Y proteins. A, purified His-tagged INV-E and His-tagged INV-E:C294Y proteins (0.5 μg of protein lane−1) were separated on a12% SDS-PAGE gel and detected by silver staining. B, shown is the effect of thiol-modifying reagents on the invertase activity of recombinant INV-E and INV-E:C294Y. The specific activities of the recombinant INV-E and INV-E:C294Y proteins without the reagent (none) were 32.8 and 40.8 mmol of product min−1 mg−1 protein, respectively, and were normalized (= 100%). DTT, dithiothreitol; pCMB, p-chloromercuribenzoate.