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. 2010 Mar 19;285(20):15296–15301. doi: 10.1074/jbc.M109.085035

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FIGURE 2. — A, LRET lifetimes for ΔNR1*Thr-396Histag/ΔNR1*T396C-Th:ΔNR2A* labeled with terbium chelate:(Ni-NTA)₂Cy3 as measured by the sensitized emission of acceptor at 575 nm in the presence of saturating concentrations of agonists. No LRET signal was observed. B, LRET lifetimes for ΔNR1*:ΔNR2A*Asn-404Histag/ΔNR2A*N404C-Th labeled with terbium chelate:(Ni-NTA)₂Cy3 as measured by the sensitized emission of acceptor at 575 nm with saturating concentrations of agonists. C, LRET lifetimes measured at 575 nm for ΔNR1*Thr-396Histag:ΔNR2A* N404C-Th labeled with terbium chelate:(Ni-NTA)₂Cy3 under saturating concentrations of agonists. D, LRET lifetimes measured at 515 nm for ΔNR1*Th-525C-Th:ΔNR2A* labeled with terbium chelate:fluorescein at saturating concentrations of agonists. For all mutants, the sensitized lifetime shown is the difference between the lifetimes obtained before and after thrombin digestion. The residuals for the lifetime fits are shown below for each measurement (the y axis is in linear scale).