FIGURE 5.

taf4 Ts⁺ and taf5 Ts⁺ mutants exhibit synthetic lethality when combined. Cells carrying the indicated alleles of taf4 and taf5 were tested for synthetic interactions by plasmid shuffle. Serial ¼ dilutions of cells carry the following: A, TAF5 plus empty vector, TAF4, or taf4 Ts⁺ alleles; B, taf5-17 + vector, TAF4, or taf4 Ts⁺ alleles; C, taf5-408 + vector, TAF4, or taf4 Ts⁺ alleles; D, taf5-10.4 + vector, TAF4, or taf4 Ts⁺ alleles, were grown on plates lacking (left, each panel) or containing 5-fluoroorotic acid (right, each panel) for 72 h at 30 °C.