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. 2010 Mar 10;285(20):14882–14889. doi: 10.1074/jbc.M109.068486

FIGURE 1.

FIGURE 1.

Isolation of κM-RIIIJ from C. radiatus venom. The asterisk in each HPLC chromatogram indicates the location of the fraction containing the peptide κM-RIIIJ. All of the elution buffers had 0.1% trifluoroacetic acid. A, the venom extract was chromatographed in a preparative Vydac C18 column eluted with 2–100% B90. B, the peak containing κM-RIIIJ in A was subfractionated using an analytical Vydac C18 column and an elution gradient of 10–15% B90 at a flow rate of 0.2%B90/min. C, the peak containing κM-RIIIJ in B was subfractionated using the same gradient as in B. D, the peak containing κM-RIIIJ in C was subfractionated using the gradient of 7–15% B90 at a flow rate of 0.2% B90/min.