FIGURE 3.

FRET interaction between TRPA1 and TRPV1 on the plasma membrane. Images were acquired using TIRF microscopy, which shows fluorophores only on plasma membranes. Images were adjusted to the same pixel range before and after photobleaching. A, representative images for FRET on the plasma membrane between different proteins/channels. Images were collected before (pre bleach) and after bleaching (post bleach). Transfected expression constructs are indicated. The top row of images represents positive and negative controls. The bottom row of images represents the interaction between a variety of combinations of the TRPV1 and TRPA1 channels. B, FRET interaction efficiency calculated for TRPV1-CFP and TRPA1-CFP with either YFP-M, α-ENaC, TRPA1-YFP, or TRPV1-YFP. Statistical analyses used unpaired t tests; *, p < 0.05. Numbers of analyzed cells for each pair of expression constructs are indicated. Error bars, S.E.