FIGURE 4.
Mustard oil activates single channel currents in TRPA1- and TRPA1-TRPV1-expressing CHO cells. All recordings were performed in cell-attached configuration in voltage clamp mode. Holding potential (Vh) was −60 mV in A and B and +60 mV in C. MO was applied for 30 s. Representative single current recordings show the effect of MO (25 μm) application on untransfected CHO cells (A, top) and TRPA1-transfected CHO cells (A, bottom). The start of MO application is indicated by an arrow. Expanded scales of the traces generated by MO applied to TRPA1-containing (B) and TRPA1-TRPV1 co-transfected (C) CHO cells are illustrated. c, the closed state. Multiple conductance states (Y1–Y3) are marked. The prolonged all-point amplitude histogram for a TRPA1-TRPV1 co-expressing cell is shown in C (right).