FIGURE 1.

Schematic representation of an interaction network. A schematic interaction network as used in the interacting protein prediction methodology is shown with a simple model system where the protein of interest, A, is linked to three “second degree” interactors, B, C, and D, via four intermediates, W–Z. In this system, all individual interactions have been shown experimentally; however, the idea that A is in complex with B, C, or D is purely theoretical. As such, B, C, and D are predicted, or candidate, interactors of A; these proteins represent the focus of the interacting protein methodology.