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Localization of mannosyltransferases in the wild-type or Δsvp26 cells. Mannosyltransferases Mnn2, Mnn5, Mnn1, and Och1 were tagged with three tandem copies of HA at the C terminus by homologous recombination using integration plasmids either in the wild-type or Δsvp26 strains. HA-tagged mannosyltransferases were visualized by immunofluorescence straining with mouse anti-HA mAb. Mnn9 was visualized with rabbit anti-Mnn9 antiserum.
