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. 2010 Mar 17;285(20):15420–15429. doi: 10.1074/jbc.M109.086272

FIGURE 6.

FIGURE 6.

Localization of the chimera mannosyltransferases in the presence or absence of Svp26. Top, localization of the chimera proteins composed of Ktr3 and Mnn1. In the ΔSVP26 strain, the chimera with the lumenal domain of Ktr3, but not the chimera with the lumenal domain of Mnn1, showed mislocalization to the ER. Introduction of the SVP26 gene on a CEN plasmid recovered the normal Golgi distribution of the Mnn1-Ktr3 chimera. Bottom, localization of the chimera proteins composed of Mnn2 and Mnn1. Similarly to the results at the top, the chimera with the lumenal domain of Mnn2, but not the chimera with the lumenal domain of Mnn1, showed mislocalization to the ER. N and C indicate the N terminus and C terminus of the chimeras, respectively.