FIGURE 1.

Thioredoxin activity of C-terminal WT and mutated CDSP32. Reduction of insulin (1 g·liter⁻¹) by 5 μm C-terminal CDSP32 was monitored as the increase in turbidity at 650 nm due to precipitation, in 0.1 m K₂HPO4, 2 mm EDTA, pH 7.0, in a final volume of 700 μl. The reaction was started by adding 0.7 mm DTT.