Table 4. Crystallization information for 14 isomorphous atomic resolution structures of HCA II.

	Crystallization conditions
PDB code	Method	Drop composition	Protein solution	Precipitant solution
1moo	Hanging-drop vapor diffusion	5 µl protein solution + 5 µl precipitant solution	10 mg ml−1 in 50 mM Tris–HCl pH 7.8, 1 mM HgCl2	2.3–2.5 M (NH4)2SO4 in 50 mM Tris–HCl pH 7.8, 1 mM HgCl2
2eu2	Hanging-drop vapor diffusion	5 µl protein solution + 5 µl precipitant solution	∼15 mg ml−1 in 50 mM Tris–HCl pH 7.8	50 mM Tris–HCl pH 7.8, 2.6 M (NH4)2SO4
2foq	Hanging-drop vapor diffusion	5 µl protein solution + 5 µl precipitant solution	10 mg ml−1 in 1 mM methylmercuric acetate, 50 mM Tris–sulfate pH 8.0	2.5 M (NH4)2SO4, 50 mM Tris–sulfate pH 7.7
2fos
2fou
2fov
2ili	Hanging-drop vapor diffusion	5 µl protein solution + 5 µl precipitant solution	∼25 mg ml−1 in 50 mM Tris–HCl pH 8.2	100 mM Tris–HCl pH 7.8, 1.15 M sodium citrate
2nng	Hanging-drop vapor diffusion	5 µl protein solution + 5 µl precipitant solution	10 mg ml−1 in 1 mM methylmercuric acetate, 50 mM Tris–sulfate pH 8.0	2.5 M (NH4)2SO4, 50 mM Tris–sulfate pH 7.7
2nno
2nns
2nnv
3d92	Hanging-drop vapor diffusion	5 µl protein solution + 5 µl precipitant solution		1.3 M sodium citrate, 100 mM Tris–HCl pH 7.8
3d93
3k34	Hanging-drop vapor diffusion	1:1 mixture of protein and precipitant solution	10 mg ml−1 in water	3 M (NH4)2SO4 in 50 mM Tris–HCl pH 7.5 and 2 mM pHMB