Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Feb 25;19(11):2113–2122. doi: 10.1093/hmg/ddq088

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2010. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Electron microscopy of liposome tubulation. (A and B) Negative stain of OPA1-S1 reconstituted with liposomes mimicking the mitochondrial inner membrane lipid. (A) A low magnification view showing OPA1-S1 tubular structures with diameters in the range of 50 to 150 nm. In (B), a higher magnification view shows an individual membrane tubule with a periodic arrangement of OPA1-S1 on the lipid substrate. The inset represents the Fourier transform of a segment of the displayed tube indicating molecular order mainly along the long axis of the tube. (C) Cryo-TEM image of vesicle preparations prior to the addition of OPA1-S1. Frozen–hydrated vesicles appear as a mixture of unilamellar and multilamellar vesicles of varying diameters. (D) Cryo-TEM of the vesicle preparation after the addition of OPA1-S1. OPA1-S1 reconstitutes into tubular structures with OPA1-S1 molecules decorating the surface of the lipid tubules. (E) Negative stain of OPA1 mixed with liposomes, showing that OPA1 assembles on the tubular as well as non-tubular (arrow) portions of the liposome. (F) Negative-stained preparation of an OPA1/liposome reaction, showing patches of OPA1 arrays. Scale bars: (A) 500 nm; (B and D) 50 nm; (C) 200 nm; (E and F) 100 nm.