Figure 7.

Loss of collagen-binding function of E113K-DDR2. (A) SDS–polyacrylamide gel electrophoresis of purified WT-DDR2-Fc, E113K-DDR2-Fc and W52A-DDR2-Fc. A Coomassie-blue-stained gel (7.5% gel) is shown. The positions of molecular weight markers (in kDa) are indicated. (B and C) Solid phase binding assays with recombinant wild-type DDR2-Fc, E113K-DDR2-Fc or W52A-DDR2-Fc. Recombinant DDR2 ectodomain proteins were added for 3 h at room temperature to 96-well plates coated with collagen I (B) or collagen II (C) at 10 µg/ml. Shown are the means ± SEM of three independent experiments, each performed in duplicates.