Figure 3.

Tcap KO mouse muscles reveal atrophic scattered fibers with central nucleation. Isopentane-frozen gastrocnemius muscles from WT (A–C) and Tcap KO (D–F) mice were stained with hematoxylin and eosin (A, D) or NADH (B, E), showing scattered atrophic fibers in the Tcap KO mice, with coarse basophilic inclusions in some small fibers and an increase in the number of centrally nucleated fibers (D, inset). Toluidine blue staining of Epon-embedded quadriceps muscle also reveals atrophic fibers in Tcap KO mice (C, F). Scale Bar = 100 µm in A and D, and 200 µm in B, C, E and F.