Figure 3.

hSOD1^mus tg mice develop weakness and abnormalities in motor function. (A and B) Normal non-tg mice extend their hind legs and splay their digits (A) when lifted by the tail, whereas age-matched hSOD1^mus tg mice (8–10 months old, line 112) are weak and cannot extend their hind legs or forelimbs (B). (C and D) When climbing on a steel wire incline, normal non-tg mice assume a broad gait and grip the bars with their paws, whereas age-matched hSOD1^mus tg mice (12–15 months old, line 25) cannot extend their legs, and their paws are unable to grip the bars. (E) hSOD1^mus tg mice expressing mSOD1 variants have significantly decreased activity in a running wheel compared with age-matched non-tg mice. Values are mean ± SEM (different lines for each construct are grouped). Asterisk indicates significant difference (P < 0.05) from control. (F) hSOD1^mus tg mice expressing mutant and wild-type hSOD1 variants have significantly decreased ability to hang from a wire compared with age-matched non-tg mice. Values are mean ± SEM (different lines for each construct are grouped). Asterisk indicates significant difference (P < 0.05) from control.