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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: J Immunol. 2008 Sep 1;181(5):2961–2972. doi: 10.4049/jimmunol.181.5.2961

FIGURE 6.

FIGURE 6

B cell subset sensitivity to anti-CD79-mediated depletion correlates with CD79β expression pattern. MRL/lpr-Thy1.1 female were given six weekly i.p. injections of either PBS or 0.5 mg each of anti-CD79α and Anti-CD79β Abs (AB) (Table II, Expt. IV; n = 5 mice/group). At week 7, composition of splenic B cells was analyzed by staining cells with Abs against CD19, CD21, CD23, and CD79β or CD19 and IgD. CD19+ splenic B cells were gated further for follicular (FO), marginal zone (MZ), and newly formed (NF) B cells based on their CD21 and CD23 expression levels. A–C, Percentage and absolute cell number of B cell subsets. Shown are means and SEM. D, Low expression of CD79β in newly formed B cells (CD19+CD21lowCD23low). Shown are representative CD79β staining pattern of splenic FO, MZ, and NF B cells from control. NC (negative control) were splenic lymphocytes incubated with anti-CD19, anti-CD21, and anti-CD23 but not with Anti-CD79β Ab. Significant differences (*, p < 0.05; **, p < 0.01) were between AB and control-treated group.