Figure 4. Effects of chronic treatment with EUK-189 or EUK-207 on reactive oxygen species (ROS) levels in brain homogenates.
At the end of the 6-month treatment, brain ROS content was quantified by incubating brain homogenates with 2′-7-dichlorofluorescein diacetate (DCFH-DA). ROS content was also determined in brain homogenates from 16 month-old control mice. Results were expressed as percentage of 16 month value. Shown are means ± SEM of 8-10 mice. One-way ANOVA indicated that the effect of age was highly significant (*p <0.001 vs. 16 month-old mice), as was the effect of EUK-189 and EUK-207 (**p <0.001 vs. vehicle control).