Figure 1.

Generation of Notch1^12f/lbd embryos. (A) Diagram of the Notch1^12f and Notch1^lbd alleles. (B) Diagram of mouse Notch1 EGF repeats in Notch1^12f and Notch1^lbd extracellular domains. The EGF repeats with putative O-fucosylation sites are shaded in gray and the mutation in EGF12 is shown. (C) Genotyping by PCR from E9.5 yolk sac DNA of a litter from a Notch1^12f/12f × Notch1^+/lbd cross. Primers 5F and 6R detect the Notch1^12f and Notch1⁺ alleles, primers 5F and 9R detect the Notch1^lbd allele.