Table 1.
Primer sets for the PCR amplification and sequencing of vector-borne infective agents used in the study
| Agent | Primers | Reference(s) |
|---|---|---|
| Babesia spp. | PIRO-A: 5'-AAT ACC CAA TCC TGA CAC AGG G-3' PIRO-B: 5'-TTA AAT ACG AAT GCC CCC AAC-3' |
[44] |
| Anaplasma spp./Ehrlichia spp. | EHR16SD: 5'-GGT ACC YAC AGA AGA AGT CC-3' EHR16SR: 5'-TAG CAC TCA TCG TTT ACA GC-3' |
[45,46] |
| Hepatozoon spp. | HEP-F: 5'-ATA CAT GAG CAA AAT CTC AAC-3' HEP-R: 5'-CTT ATT ATT CCA TGC TGC AG-3' |
[47,48] |
| Leishmania spp. | ITS-219F: 5'-AGC TGG ATC ATT TTC CGA TG-3' ITS-219R: 5'-ATC GCG ACA CGT TAT GTG AG-3' |
[49] |