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. 2010 Mar 26;11:44. doi: 10.1186/1471-2202-11-44

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Copyright ©2010 McHugh et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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BV-2 microglia express GPR18 mRNA and GPR18 receptors. (A) Gel electrophoresis of BV-2 microglia and HEK293-GPR18 RT-qPCR products. RT-qPCR products were collected from the RT-qPCR run, loading buffer was added to the samples, and samples were run on a 2% agarose gel. No template control (NTC) and a control without reverse transcription (NRT) were used as controls. (B) Representative qPCR amplification curves showing the different amounts of mRNAs for GPR18 in primary microglia and BV-2 cells; n = 3.