Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 May 6;6(5):e1000939. doi: 10.1371/journal.pgen.1000939

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

(A) Schematic maps of origin regions of E. coli chromosome and V. cholerae chromosome I. The regions are very similar except that the V. cholerae origin (oriI) has an extra DnaA box (R6). Other details are described in Figure 5A. The tau and I sites of the E. coli origin (oriC) that also bind DnaA are yet to be described in oriI. The two GATC sites studied here for their methylation states are shown by vertical arrows. (B) The WT either had oriC (MG1655) or oriI (MG1655ΔoriC::oriI). The GATC sites probed were located either within the origins (oriC or oriI) or external to the origin (ext) at about 300 kb away. The same ext marker was used for both the strains. The numbers below the figure show the percent of hemimethylated DNA at the origins. N.A. stands for ‘not applicable’; in these lanes the DNA being from a dam mutant is unmethylated, and is all cleaved both at the origin and the external markers. An uncharacterized cross-reacting band appears in the dam mutants only (arrow head).