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. 2010 May 6;6(5):e1000897. doi: 10.1371/journal.ppat.1000897

Figure 4. PDEH deletion abolishes surface dependency during pathogenesis.

Figure 4

Appressorium formation assays on inductive and non-inductive surfaces. (A) Wild-type, pdeHΔ or pdeLΔ conidia were inoculated on inductive (plastic cover slips) or non-inductive surfaces (GelBond membrane) and assessed for appressoria formation after 12 h. In addition to forming appressoria on inductive surface, the pdeHΔ strain elaborated melanized appressoria on non-inductive surface too. The arrows highlight extremely short or negligible germ tubes formed by the pdeHΔ conidia on inductive plastic cover slips. Scale bar = 10 micron. (B) Bar graph depicting the efficiency of appressorium formation in the pdeHΔ, pdeLΔ and wild type on inductive (black bar) or non-inductive surface (white bar). Values represent mean ± S.E from three independent replicates involving 1000 conidia per sample.